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Distribution of preprovasopressin mRNA in the rat central nervous system
Author(s) -
Hallbeck Martin,
Hermanson Ola,
Blomqvist Anders
Publication year - 1999
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19990823)411:2<181::aid-cne2>3.0.co;2-1
Subject(s) - vasopressin , biology , diagonal band of broca , neuroscience , supraoptic nucleus , locus coeruleus , median preoptic nucleus , stria terminalis , hypothalamus , zona incerta , neuropeptide , suprachiasmatic nucleus , central nervous system , circumventricular organs , nucleus , dentate gyrus , medicine , endocrinology , subfornical organ , cholinergic neuron , angiotensin ii , biochemistry , receptor , blood pressure
Vasopressin released in the central nervous system has been shown to be involved both in homeostatic mechanisms (e.g., water balance, thermoregulation, cardiovascular regulation, metabolism, and antinociception) and in higher brain functions (e.g., social recognition and communication, and learning and memory). Many nuclear groups have been proposed to synthesize vasopressin, but available data are conflicting. We have used a sensitive in situ hybridization technique to identify the distribution of the neurons that may be the origin of the vasopressin in the central nervous system of the male Sprague‐Dawley rat. Vasopressin mRNA‐expressing neurons were most abundant in the hypothalamus (e.g., the paraventricular, supraoptic, and suprachiasmatic nuclei) but were also seen in the medial amygdaloid nucleus, the bed nucleus of stria terminalis, and the nucleus of the horizontal diagonal band. Previously unreported vasopressinergic neurons were seen in the entorhinal and piriform cortices, the ventral lateral portion of the parabrachial nucleus, the pedunculopontine nucleus, and the rostral part of the ventral periaqueductal gray matter and the adjacent portion of the mesencephalic reticular nucleus. Vasopressin mRNA expression suggestive of neuronal labeling was seen in the pyramidal layer of the CA1–3 fields and the dentate gyrus of the hippocampus. In addition, vasopressin mRNA expression, probably representing axonal mRNA, was detected over the hypothalamopituitary tract. No or insignificant preprovasopressin mRNA expression was present in the cerebellum, locus coeruleus, subcoeruleus, or the spinal cord. These findings provide novel information on the distribution of vasopressin neurons that are important for our understanding of how vasopressin acts in the brain. J. Comp. Neurol. 411:181–200, 1999. © 1999 Wiley‐Liss, Inc.