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Autoradiographic and in situ hybridization localization of corticotropin‐releasing factor 1 and 2 receptors in nonhuman primate brain
Author(s) -
Sánchez M. Mar,
Young Larry J.,
Plotsky Paul M.,
Insel Thomas R.
Publication year - 1999
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19990607)408:3<365::aid-cne5>3.0.co;2-n
Subject(s) - biology , neocortex , neuroscience , receptor , hippocampal formation , medicine , endocrinology , circumventricular organs , central nervous system , biochemistry
Two different corticotropin‐releasing factor (CRF) receptors, CRF 1 and CRF 2 , have been identified in rat and human brain. Although the two receptor subtypes show a markedly different distribution in the rat brain, their distribution in the primate brain has not been described previously. In this study, the neuroanatomic distribution of CRF 1 and CRF 2 receptor binding sites in rhesus monkey ( Macaca mulatta ) was assessed by using iodine 125 ([ 125 I)‐Tyr 0 ]‐sauvagine with or without the selective CRF 1 receptor antagonist CP‐154,526–1. Radiolabeled human cRNA probes were used to map the distribution of the two receptor mRNAs with in situ hybridization. Both CRF 1 and CRF 2 receptors were found in the pituitary and throughout the neocortex (especially, in prefrontal, cingulate, striate, and insular cortices), amygdala, and hippocampal formation of the monkey brain. This is in contrast to the distribution of these receptors reported in the rat brain, in which generally only the CRF 1 receptor is found in the pituitary and neocortex. These results suggest that, in primates, both CRF 1 and CRF 2 receptors may be involved in mediating the effects of CRF on cognition, behavior, and pituitary‐adrenal function. The presence of CRF 1 (but not CRF 2 ) receptors within the locus coeruleus, cerebellar cortex, nucleus of the solitary tract, thalamus, and striatum and of CRF 2 (but not CRF 1 ) receptors in the choroid plexus, certain hypothalamic nuclei, the nucleus prepositus, and the nucleus of the stria terminalis suggests that each receptor subtype also may have distinct functional roles within the primate central nervous system. J. Comp. Neurol. 408:365–377, 1999. © 1999 Wiley‐Liss, Inc.