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Immunochemical localization of calretinin in the retina of the turbot ( Psetta maxima ) during development
Author(s) -
Doldan M.J.,
Prego B.,
De Miguel Villegas E.
Publication year - 1999
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19990419)406:4<425::aid-cne1>3.0.co;2-1
Subject(s) - calretinin , retina , inner plexiform layer , biology , outer plexiform layer , ganglion , ganglion cell layer , microbiology and biotechnology , calbindin , anatomy , neuroscience , immunohistochemistry , immunology
The expression of the calcium‐binding protein calretinin was analysed by immunohistochemistry techniques in the retina of turbot ( Psetta maxima ) from embryonic to juvenile stages. Calretinin immunoreactivity was first detected in retinae from newly hatched larvae, in which the anlage of the inner plexiform layer and a subset of amacrine and ganglion cells displayed a faint immunolabelling. First appearance of photoreceptors during larval life coincided with an increase in the intensity of the labelling. During subsequent larval development, the expression of calretinin affected distinctive retinal components. The inner plexiform layer, optic fiber layer, and a population of amacrine and ganglion cells were invariably labelled. Occasional bipolar cells were labelled at the end of the larval period. By metamorphosis, calretinin is sequentially expressed in horizontal cells, and bipolar immunoreactive cells become numerous. The pattern of calretinin immunoreactivity of the inner plexiform layer changes from the larval to juvenile period. In all cases, calretinin immunoreactivity exhibited variations between the peripheral retina, which contains the most recently differentiated retinal components, and the remainder of the differentiated retina. Our results suggest that the progressive expression of calretinin in the turbot retina appears associated with some degree of neuronal differentiation. Once the definitive pattern of calretinin immunoreactivity is established in the turbot retina, both similarities and differences with the calretinin location in the retina of other vertebrates can be demonstrated. J. Comp. Neurol. 406:425–432, 1999. © 1999 Wiley‐Liss, Inc.