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Clonal mixing, clonal restriction, and specification of cell types in the developing rat olfactory bulb
Author(s) -
Reid,† Christopher B.,
Liang,† Ivan,
Walsh Christopher A.
Publication year - 1999
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19990105)403:1<106::aid-cne8>3.0.co;2-s
Subject(s) - biology , olfactory bulb , progenitor cell , neurogenesis , neocortex , progenitor , embryonic stem cell , cell type , microbiology and biotechnology , rostral migratory stream , genetics , stem cell , neuroscience , cell , central nervous system , subventricular zone , gene
To understand the clonal relationship of various olfactory bulb (OB) cell types, OB progenitor cells were infected at embryonic day (E) 14, E15, and E17 with retroviral libraries encoding alkaline phosphatase or β‐galactosidase. After survival to postnatal day 10–15, sibling relationships were identified by polymerase chain reaction DNA amplification of distinct sequences in the retroviral constructs. Within the OB, clonal progeny dispersed widely in all directions. In sharp contrast, however, clonal dispersion between the OB and neocortex was not observed, although occasional clonal dispersion between the OB and pyriform and hippocampal regions could not be excluded. Most clones (84%) contained a single cell type, especially after E17 injections, suggesting the existence of either restricted precursors, or multipotential progenitors instructed by a restricted cellular environment. Mixed OB clones (16%) contained multiple cell types in the OB, or occasionally glial or neuronal cells outside the OB, demonstrating the existence of multipotential OB progenitors, likely at a stage before formation of the olfactory rostral migratory stream. Surprisingly, OB glial cells were not labeled, suggesting distinct lineages or perhaps distinct migratory paths for glia and neurons into the OB. A hierarchical cell lineage is proposed that involves a multipotential progenitor that gives rise to potentially more limited progenitors. J. Comp. Neurol. 403:106–118, 1999. © 1999 Wiley‐Liss, Inc.