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Chemical anatomy of the Macaque monkey olfactory bulb: NADPH‐diaphorase/nitric oxide synthase activity
Author(s) -
Alonso José R.,
Porteros A.,
Crespo C.,
Arévalo R.,
Briñón J.G.,
Weruaga E.,
Aijón J.
Publication year - 1998
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19981221)402:3<419::aid-cne9>3.0.co;2-c
Subject(s) - olfactory bulb , biology , granule cell , nitric oxide synthase , macaque , axon , nadph dehydrogenase , anatomy , nicotinamide adenine dinucleotide phosphate , microbiology and biotechnology , nitric oxide , central nervous system , biochemistry , dentate gyrus , endocrinology , neuroscience , oxidase test , enzyme
The distribution and the morphology of nicotinamide adenine dinucleotide phosphate (NADPH)‐diaphorase (ND)‐activeneuronal nitric oxide synthase (NOS)‐immunoreactive neuronsfibers were studied in the olfactory bulb of three species of primates, i.e., the cynomolgus macaque monkey ( Macaca fascicularis ), the Japanese macaque monkey ( Macaca fuscata), and the pig‐tail macaque monkey ( Macaca nemestrina ). The ND staining was carried out by means of a direct histochemical method with β‐NADPH as cosubstratenitro blue tetrazolium as chromogen. The NOS immunostaining was carried out by using a polyclonal antibodythe avidin‐biotin peroxidase method. Similar results were found in the three species, where a distinct distribution pattern of ND/NOS‐stained neuronsfibers was observed. All olfactory fibers demonstrated ND‐positive labeling but they were NOS‐immunonegative. In the superficial modulatory area of the olfactory bulb, a few weakly ND‐NOS‐positive periglomerular cells, stellate cells,and darkly stained superficial short‐axon cells were observed. In the inframitral layers, granule cells, deep stellate cells, and deep short‐axon cells were distinguished. Short‐axon cells had oriented morphologiesspiny dendrites. Many thick, varicose ND/NOS‐stained fibers identified as centrifugal fibers were observed in the white matter, granule cell layer, internal plexiform layer, mitral cell layer, and external plexiform layer. This distribution of ND activityNOS immunoreactivity showed similarities to and differences from what has been reported in the olfactory bulb of macrosmatic mammals including rodents (rat, mouse, and hamster)insectivores (hedgehog). These data confirm that the complexity of the ND/NOS staining in the olfactory bulb of one species correlates with the importance of olfaction in the biology of such species. J. Comp. Neurol. 402:419–434, 1998. © 1998 Wiley‐Liss, Inc.