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Cellular distribution of adenosine A 2A receptor mrna in the primate striatum
Author(s) -
Svenningsson P.,
Le Moine C.,
Aubert I.,
Burbaud P.,
Fredholm B.B.,
Bloch B.
Publication year - 1998
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19980921)399:2<229::aid-cne6>3.0.co;2-2
Subject(s) - adenosine a2a receptor , nucleus accumbens , biology , medium spiny neuron , striatum , adenosine a1 receptor , adenosine , basal ganglia , medicine , adenosine receptor , dopamine receptor d1 , endocrinology , dopamine receptor d2 , receptor , dopamine , biochemistry , central nervous system , agonist
The cellular expression of adenosine A 2A receptor mRNA in the adult monkey and human striatum was examined by using single and double in situ hybridization with ribonucleotide probes. Analysis on adjacent sections demonstrated a homogeneous overlapping expression of adenosine A 2A receptor and preproenkephalin A mRNAs throughout nucleus caudatus, putamen, and nucleus accumbens. By contrast, high expression of preproenkephalin A mRNA but no expression of adenosine A 2A receptor mRNA was found in the nucleus basalis of Meynert. Double in situ hybridization demonstrated an extensive colocalization of adenosine A 2A receptor and preproenkephalin A mRNAs in approximately 50% of the medium‐sized spiny neurons of the monkey nucleus caudatus, putamen, and nucleus accumbens. A small number of neurons (4–12%) that contained adenosine A 2A receptor mRNA but not preproenkephalin A mRNA was found along the ventral borders of the striatum. Virtually all adenosine A 2A receptor mRNA‐containing neurons co‐expressed dopamine D 2 receptor mRNA, whereas only very few adenosine A 2A receptor mRNA containing neurons co‐expressed dopamine D 1 receptor or substance P mRNAs. In addition, a sub‐population of adenosine A 2A receptor mRNA‐expressing neurons that also contained preproenkephalin A mRNA was found in the septum in monkeys. These results demonstrate that there is a high expression of adenosine A 2A receptor mRNA in the primate striatum that is extensively co‐localized with dopamine D 2 receptor and preproenkephalin A mRNAs. It is concluded that adenosine A 2A receptors are likely to be important for the parallel organization of primate striatal neurotransmission and that these receptors could be a target for drug therapy in Parkinson's disease. J. Comp. Neurol. 399:229–240, 1998. © 1998 Wiley‐Liss, Inc.