Premium
Concomitant expression of genes encoding integrin αvβ5 heterodimer and vitronectin in growing parallel fibers of postnatal rat cerebellum: A possible role as mediators of parallel fiber elongation
Author(s) -
Murase ShinIchi,
Hayashi Yokichi
Publication year - 1998
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19980727)397:2<199::aid-cne4>3.0.co;2-w
Subject(s) - biology , cerebellum , parallel fiber , microbiology and biotechnology , integrin , vitronectin , granule cell , neurite , granule (geology) , receptor , neuroscience , biochemistry , purkinje cell , in vitro , central nervous system , dentate gyrus , paleontology
External granule cells in the premigratory zone and the upper molecular layer of neonatal rat cerebellum elongate their neurites (parallel fibers) bidirectionally before and during migration into the internal granular layer. In the present study, it is shown that integrin αvβ5 heterodimer (INTαvβ5) is expressed in parallel fibers in these layers at postnatal days 3–20, but not in migrating granule cells or mature parallel fibers. Vitronectin (VN), the dominant ligand for INTαvβ5, was concomitantly detected in the premigratory zone and the upper molecular layer during this period. Several other subunits including α1–6 and β1–4 were not detected. When granule cells were prepared from postnatal cerebella and cultured for a few days, the parallel fibers elongated well in response to VN, but the granule cells did not migrate on VN. This fiber elongation was specifically inhibited by both anti‐INTαvβ5 antibody and peptides containing Arg‐Gly‐Asp (RGD), a sequence responsible for cell adhesion mediated by VN. Neither control integrin antibody against integrin αvβ3 heterodimer nor control peptides containing Arg‐Gly‐Glu (RGE) showed an inhibitory effect on fiber elongation. These observations strongly suggest that the INTαvβ5 VN receptor plays a role in the elongation of parallel fibers from granule cells during cerebellar histogenesis, but its expression is not required for their maintenance or granule cell migration. INTαvβ5 could be considered as a new marker of parallel fibers during cerebellar development. J. Comp. Neurol. 397:199–212, 1998. © 1998 Wiley‐Liss, Inc.