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Insulin‐like growth factor‐I binds in the inner plexiform layer and circumferential germinal zone in the retina of the goldfish
Author(s) -
Boucher ShayneEmile M.,
Hitchcock Peter F.
Publication year - 1998
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19980511)394:3<395::aid-cne10>3.0.co;2-o
Subject(s) - retina , biology , neurogenesis , inner plexiform layer , microbiology and biotechnology , outer plexiform layer , insulin like growth factor , growth factor , receptor , endocrinology , retinal , muller glia , medicine , neuroscience , progenitor cell , biochemistry , stem cell
Results of the previous study suggest that insulin‐related peptides regulate proliferation of retinal progenitors in the adult goldfish. Because of their known roles in retinal neurogenesis, we have chosen to focus future studies on insulin‐like growth factor I (IGF‐I) and the IGF‐I receptor. In the study described here, we characterized the spatial distribution and specificity of IGF‐I binding sites in the retina of the adult goldfish by performing receptor‐binding autoradiography with [ 125 I]‐IGF‐I alone and with unlabeled IGF‐I‐related molecules (IGF‐I, IGF‐II, insulin, and des‐[1‐3]‐IGF‐I) as competitive inhibitors of [ 125 I]‐IGF‐I binding. The results of these experiments show that IGF‐I binds in two locations in the retina of the adult goldfish, within the inner plexiform layer of the differentiated retina and the circumferential germinal zone. The competition experiments suggest that [ 125 I]‐IGF‐I binds at sites specific for IGF‐I, and that both IGF‐I receptors and IGF‐I binding proteins are present in the retina. J. Comp. Neurol. 394:395–401, 1998. © 1998 Wiley‐Liss, Inc.