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Long‐term effects of neonatal axoplasmic transport attenuation on the organization of the rat's trigeminal system
Author(s) -
Chiaia Nicolas L.,
BennettClarke Carol A.,
Crissman Robert S.,
Zhang Shujie,
Rhoades Robert W.
Publication year - 1997
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19970505)381:2<219::aid-cne8>3.0.co;2-6
Subject(s) - axoplasmic transport , infraorbital nerve , retrograde tracing , trigeminal ganglion , chemistry , anatomy , parvalbumin , horseradish peroxidase , ganglion , neuroscience , central nervous system , sensory system , biology , biochemistry , enzyme
Abstract The current study examined the long‐term effects of infraorbital nerve (ION) axoplasmic transport attenuation with vinblastine on the organization of trigeminal (V) primary afferents and central vibrissae‐related patterns. Retrograde tracing and single unit recording were used to evaluate the innervation of vibrissae follicles in adult ( P > 60) rats that sustained application of vinblastine to the ION at birth. Single units recorded from vinblastine‐treated animals yielded responses to deflection of a single vibrissa, and a significantly ( P < 0.001) higher percentage of these cells (85.7%) showed rapidly adapting responses compared with normal rats (42.2%). Retrograde tracing revealed a qualitatively and normal distribution of V ganglion cells innervating A‐row and E‐row vibrissae follicles in vinblastine‐treated rats. Transganglionic tracing with horseradish peroxidase (HRP) demonstrated a qualitatively and quantitatively normal somatotopic organization of vibrissae follicle input to V nucleus principalis (PrV) and V subnucleus interpolaris (SpI) in the vinblastine‐treated animals. Despite the nearly normal mapping of V ganglion cell axons onto the vibrissae follicles and brainstem, staining for either cytochrome oxidase (CO) or parvalbumin failed to reveal vibrissae‐related patterns in PrV, SpI, or the magnocellular portion of V subnucleus caudalis in these animals. Labelling of thalamocortical afferents with HRP and staining for CO also failed to reveal a cortical vibrissae‐related pattern in the vinblastine‐treated rats. The present results indicate that although transient attenuation of axoplasmic transport with vinblastine has limited effects on the peripheral and central projections of surviving V primary afferents, it permanently disrupts the normal development and maintenance of central vibrissae‐related patterns. J. Comp. Neurol. 381:219‐229, 1997. © 1997 Wiley‐Liss, Inc.