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Immunocytochemistry and distribution of parabrachial terminals in the lateral geniculate nucleus of the cat: A comparison with corticogeniculate terminals
Author(s) -
Erişir Alev,
Van Horn Susan C.,
Bickford Martha E.,
Sherman S. Murray
Publication year - 1997
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19970127)377:4<535::aid-cne5>3.0.co;2-3
Subject(s) - biology , choline acetyltransferase , parabrachial nucleus , brainstem , neuroscience , anatomy , hypoglossal nucleus , inferior colliculus , glutamatergic , nucleus , cholinergic , glutamate receptor , biochemistry , receptor
We used immunohistochemistry in cats to demonstrate the presence of brain nitric oxide synthase (BNOS) in cholinergic fibers within the A‐laminae of the lateral geniculate nucleus. We used a double labeling procedure with electron microscopy and found that all terminals labeled for choline acetyltransferase (ChAT) in the geniculate A‐laminae were double labeled for BNOS. Also, some interneuron dendrites, identified by labeling for γ‐aminobutyric acid (GABA), contained BNOS, but relay cell dendrites did not. We then compared parabrachial and corticogeniculate terminals, identifying the former by BNOS/ChAT labeling and the latter by orthograde transport of biocytin injected into cortical area 17, 18, or 19. All corticogeniculate terminals and most BNOS‐ or ChAT‐positive brainstem terminals displayed RSD morphology, whereas some brainstem terminals exhibited RLD morphology. However, parabrachial terminals were larger, on average, then corticogeniculate terminals. We also found that parabrachial terminals were located both inside and outside of glomeruli, and they always contacted relay cell dendrites proximally among retinal terminals (the retinal recipient zone). In contrast, the cortical terminals were limited to peripheral dendrites (the cortical recipient zone). Thus, little if any overlap exists in the distribution of parabrachial and corticogeniculate terminals on the dendrites of relay cells. J. Comp. Neurol. 377:535–549, 1997. © 1997 Wiley‐Liss, Inc.