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Gephyrin accumulates at specific plasmalemma loci during neuronal maturation in vitro
Author(s) -
Colin I.,
Rostaing P.,
Triller A.
Publication year - 1996
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19961021)374:3<467::aid-cne10>3.0.co;2-s
Subject(s) - gephyrin , glycine receptor , postsynaptic potential , immunoelectron microscopy , microbiology and biotechnology , biology , neurotransmitter receptor , intracellular , spinal cord , immunocytochemistry , receptor , biophysics , neuroscience , glycine , biochemistry , genetics , antibody , amino acid , endocrinology
The distribution of glycine receptor (GlyR)‐associated gephyrin has been investigated in rat spinal cord neurons maintained in vitro by means of immunocytochemical techniques. Gephyrin, which is crucial for the stabilization of postsynaptic GlyR microdomains, is present in mature neurons at postsynaptic differentiations. With immunofluorescence, discontinous patches of gephyrin were detected within the neuronal soma of spinal cord neurons on the 1st day after plating. Subsequently, gephyrin was present at membrane areas that correspond to points of contact between cells or with the culture dish. By the 5th day, gephyrin was mostly associated with the MAP2‐positive somatodendritic compartment. With immunoelectron microscopy, gephyrin blobs detected at the earliest stages (1–3 days after plating) were found within the cytoplasm or associated with the plasma membrane. Asymmetrically immunostained intercellular contacts were only detected after 5 days, and gephyrin was found in association with clearly differentiated postsynaptic membranes at 7 days. At later stages, we observed gephyrin immunoreactivity only at some synapses. Our results suggest that gephyrin accumulates initially at the locus of cell‐to‐cell contacts involved in adhesion processes. These localizations may define hot spots for later accumulation of the GlyR and possibly other receptors. © 1996 Wiley‐Liss, Inc.