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Distribution of GABA A , GABA B , and glycine receptors in the central auditory system of the big brown bat, Eptesicus fuscus
Author(s) -
Fubara Boma M.,
Casseday John H.,
Covey Ellen,
SchwartzBloom Rochelle D.
Publication year - 1996
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19960520)369:1<83::aid-cne6>3.0.co;2-g
Subject(s) - eptesicus fuscus , gabaa receptor , biology , gabab receptor , neuroscience , glycine receptor , receptor , glycine , zoology , biochemistry , amino acid
Quantitative autoradiographic techniques were used to compare the distribution of GABA A , GABA B , and glycine receptors in the subcortical auditory pathway of the big brown bat, Eptesicus fuscus. For GABA A receptors, the ligand used was 35 S‐t‐butylbicyclophosphorothionate (TBPS); for GABA B receptors, 3 H‐GABA was used as a ligand in the presence of isoguvacine to block binding to GABA A sites; for glycine, the ligand used was 3 H‐strychnine. In the subcortical auditory nuclei there appears to be at least a partial complementarity in the distribution of GABA A receptors labeled with 35 S‐TBPS and glycine receptors labeled with 3 H‐strychnine. GABA A receptors were concentrated mainly in the inferior colliculus (IC) and medial geniculate nucleus, whereas glycine receptors were concentrated mainly in nuclei below the level of the IC. Within the IC, there was a graded spatial distribution of 35 S‐TBPS binding; the most dense labeling was in the dorsomedial region, but very sparse labeling was observed in the ventrolateral region. There was also a graded spatial distribution of 3 H‐strychnine binding. The most dense labeling was in the ventral and lateral regions and the weakest labeling was in the dorsomedial region. Thus, in the IC, the distribution of 35 S‐TBPS was complementary to that of 3 H‐strychnine. GABA B receptors were distributed at a low level throughout the subcortical auditory nuclei, but were most prominent in the dorsomedial part of the IC. © 1996 Wiley‐Liss, Inc.