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Differential expression of annexins I‐VI in the rat dorsal root ganglia and spinal cord
Author(s) -
Naciff Jorge M.,
Kaetzel Marcia A.,
Behbehani Michael M.,
Dedman John R.
Publication year - 1996
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19960506)368:3<356::aid-cne3>3.0.co;2-4
Subject(s) - annexin , ependymal cell , dorsal root ganglion , spinal cord , sensory system , microbiology and biotechnology , neuroscience , biology , central nervous system , sensory neuron , chemistry , flow cytometry
The annexins are a family of Ca 2− ‐dependent phospholipid‐binding proteins. In the present study, the spatial expression patterns of annexins I‐VI were evaluated in the rat dorsal root ganglia (DRG) and spinal cord (SC) by using indirect immunofluorescence. Annexin I is expressed in small sensory neurons of the DRG, by most neurons of the SC, and by ependymal cells lining the central canal. Annexin II is expressed by most sensory neurons of the DRG but is primarily expressed in the SC by glial cells. Annexin III is expressed by most sensory neurons, regardless of size, by endothelial cells lining the blood vessels, and by the perineurium. In the SC, annexin III is primarily expressed by astrocytes. In the DRG and the SC, annexin IV is primarily expressed by glial cells and at lower levels by neurons. In the DRG, annexin V is expressed in relatively high concentrations in small sensory neurons in contrast to the SC, where it is expressed mainly by ependymal cells and by small‐diameter axons located in the superficial laminae of the dorsal horn areas. Annexin VI is differentially expressed by sensory neurons of the DRG, being more concentrated in small neurons. In the SC, annexin VI has the most striking distribution. It is concentrated subjacent to the plasma membrane of motor neurons and their processes. The differential localization pattern of annexins in cells of the SC and DRG could reflect their individual biological roles in Ca 2− ‐signal transduction within the central nervous system. © 1996 Wiley‐Liss, Inc.