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Imaginal cell‐specific accumulation of the multicatalytic proteinase complex (proteasome) during post‐embryonic development in the tobacco hornworm, Manduca sexta
Author(s) -
Hashimoto Minako K.,
Mykles Donald L.,
Schwartz Lawrence M.,
Fahrbach Susan E.
Publication year - 1996
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19960205)365:2<329::aid-cne9>3.0.co;2-7
Subject(s) - manduca sexta , biology , microbiology and biotechnology , imaginal disc , manduca , cytoplasm , proteasome , proteolysis , biochemistry , insect , drosophila melanogaster , botany , enzyme , gene
The multicatalytic proteinase complex is a multi‐subunit, high molecular weight proteinase present in the nucleus and cytoplasm of eukaryotic cells. This catalytic complex is involved in diverse cellular functions as part of the ubiquitin proteolysis system, including non‐lysosomal proteolysis, antigen presentation, cell cycle progression, and cell proliferation, and in the programmed death of intersegmental muscles after adult eclosion in the tobacco hornworm moth, Manduca sexta . We have investigated the distribution of the multicatalytic proteinase complex in the central nervous system of this moth. At all stages of post‐embryonic development, most cell types exhibited consistent, low levels of cytoplasmic and nuclear immunoreactivity for the multicatalytic proteinase complex. High levels of cell‐specific accumulation of the complex were, however, demonstrated in abdominal neurosecretory cells and in imaginal cells in the larval brain, the larval segmental ganglia, and the developing wing discs. Imaginal cells exhibited intense immunoreactivity for the multicatalytic proteinase complex only until the onset of terminal differentiation. Intersegmental muscles undergoing programmed cell death exhibited intense cytoplasmic immunoreactivity for the multicatalytic proteinase, while persisting flight muscles and dying neurons were characterized by basal levels of staining. These staining patterns suggest that the multicatalytic proteinase of Manduca sexta serves multiple functions and is associated with the period of developmental arrest displayed by imaginal cells prior to metamorphosis. © 1996 Wiley‐Liss, Inc.