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A study of aminolevulinic acid–induced protoporphyrin IX fluorescence kinetics in the canine oral cavity
Author(s) -
Vaidyanathan Vijay V.,
Rastegar Sohi,
Fossum Theresa W.,
Flores Patricia,
van der Breggen Egbertus W.,
Egger Norman G.,
Jacques Steven L.,
Motamedi Massoud
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(2000)26:4<405::aid-lsm9>3.0.co;2-e
Subject(s) - protoporphyrin ix , pharmacokinetics , photosensitizer , protoporphyrin , chemistry , kinetics , fluorescence , tongue , buccal administration , buccal mucosa , heme , pharmacology , pathology , photodynamic therapy , medicine , oral cavity , biochemistry , dentistry , photochemistry , porphyrin , enzyme , physics , organic chemistry , quantum mechanics
Background and Objective 5‐Aminolevulinic acid–induced protoporphyrin IX is a promising photosensitizer that could enhance the spectroscopic contrast between normal and diseased oral tissues. Knowledge of the pharmacokinetics and effects on tissue type are important for diagnostic and therapeutic procedures. Study Design/Materials and Methods Dogs randomly were administered five doses of 5‐aminolevulinic acid: 5, 25, 50, 75, and 100 mg/kg. The fluorescence was recorded from buccal mucosa, gums, tongue, and facial skin using a fiberoptic probe connected to an optical multichannel analyzer. Blood samples were collected for hematologic and serum biochemical analysis. Pharmacokinetic parameters of interest were estimated using a compartmental model. Results Protoporphyrin fluorescence at all sites reached a peak in 2–6 hours, and returned to baseline in 24–31 hours, depending on the dose. Plasma protoporphyrin peaked earlier than oral tissues. Conclusion The rate of synthesis of protoporphyrin, and its conversion to heme products are dose dependent. Different tissues have different pharmacokinetic response. Lasers Surg. Med. 26:405–414, 2000. © 2000 Wiley‐Liss, Inc.