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Laser‐induced shock wave endothelial cell injury
Author(s) -
Sondén, Anders,
Svensson Bengt,
Roman Nils,
Östmark Henric,
Brismar Bo,
Palmblad Jan,
Kjellström B. Thomas
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(2000)26:4<364::aid-lsm4>3.0.co;2-t
Subject(s) - shock wave , laser , shock (circulatory) , cell injury , endothelial stem cell , biophysics , biomedical engineering , cell damage , materials science , pathology , optics , medicine , chemistry , biology , in vitro , radiology , biochemistry , physics , thermodynamics , apoptosis
Background and Objective Several laser procedures, extracorporeal lithotripsies (ESWL), and high‐velocity missile trauma generate pressure transients that are transmitted through the tissues. Despite several publications demonstrating shock wave−induced tissue injury, little is known about its pathophysiology. This study introduces an in vitro model for studying shock wave effects on endothelial cell (EC) monolayers. Study Design/Materials and Methods A Nd:YAG laser‐driven flyer‐plate technique was used to generate shock waves. Physical characteristics were determined with a pressure transducer, a high‐speed video camera, and sequential photography. Biological effects were studied with phase contrast and lightfield microscopy, computerized morphometry, immunocytochemistry, spectrophotometry, and enzyme‐linked immunosorbent assay (ELISA). Results The shock waves generated were highly reproducible. Cavitation was verified and quantified, and its extent could be varied in the vials. Exposed cultures exhibited areas with cell membrane damage and cell detachment. Release of LD was elevated ( P < 0.01) in exposed vials. The EC lesions were larger ( >P < 0.01) in cultures submitted to high vs. low extent of cavitation. Conclusion The flyer‐plate model can be used to subject cell monolayers to defined and reproducible shock waves causing immediate cell injury similar to the previously reported vascular lesions associated with ESWL, pulsed lasers, and blast trauma. With the flyer‐plate model, such lesions may be further studied on the cellular and subcellular levels. Lasers Surg. Med. 26:364–375, 2000 © 2000 Wiley‐Liss, Inc.