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Long‐term effectiveness of photodynamic therapy by using a hydrophilic photosensitizer ATX‐S10(Na) against experimental choroidal neovascularization in rats
Author(s) -
Kanai Masakazu,
Obana Akira,
Gohto Yuko,
Nagata Satoshi,
Miki Tokuhiko,
Kaneda Kenji,
Nakajima Susumu
Publication year - 2000
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(2000)26:1<48::aid-lsm8>3.0.co;2-z
Subject(s) - photodynamic therapy , choroidal neovascularization , photosensitizer , ophthalmology , medicine , retinal , retinal pigment epithelium , fundus photography , occlusion , neovascularization , retina , fluorescein angiography , fundus (uterus) , surgery , chemistry , angiogenesis , biology , photochemistry , organic chemistry , neuroscience
Background and Objective We previously demonstrated that a hydrophilic photosensitizer ATX‐S10 had a potent photodynamic effect. This study was designed to reveal the long‐term effectiveness of photodynamic therapy (PDT) with this agent in occluding choroidal neovascularization (CNV) and its selectivity in the neovascular tissue. Study Design/Materials and Methods Experimental CNV was induced by intense photocoagulation in rat eyes. Immediately or 2 hours after intravenous injection of 8 mg/kg body weight of ATX‐S10(Na), a cis isomer of ATX‐S10, eyes were irradiated by a diode laser at the radiance of 3.25–65.3 J/cm 2 Vascular occlusion was identified by fundus photography, fluorescein angiography, and histology at 1, 3, 7, 14, and 28 days after PDT. As controls, non‐neovascular eyes were subjected to PDT and similarly analyzed. Results By using the following treatment parameters, PDT with ATX‐S10(Na) successfully occluded CNV without causing occlusion of retinal capillaries for 28 days; 7.4 and 19.6 J/cm 2 immediately after dye injection and 36.7 and 65.3 J/cm 2 2 hours after injection. Although these conditions also caused occlusion of normal choriocapillaries and mild injuries of retinal vessels, retinal pigment epithelium, and photoreceptors at 1 day, retinal vessels and pigment epithelial cells recovered from damages by 28 days. No injuries were found in the inner retina. Conclusion In optimal treatment conditions, PDT with ATX‐S10(Na) can induce long‐term, selective occlusion of CNV without causing irreversible damages in the inner retina. Lasers Surg. Med. 26:48–57, 2000. © 2000 Wiley‐Liss, Inc.