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Critical temperature and heating time for coagulation damage: Implications for interstitial laser coagulation (ilc) of tumors
Author(s) -
Heisterkamp Joos,
van Hillegersberg Richard,
IJzermans Johannes N.M.
Publication year - 1999
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1999)25:3<257::aid-lsm10>3.0.co;2-v
Subject(s) - syngenic , dispase , trypan blue , collagenase , viability assay , pathology , coagulation , renal capsule , chemistry , medicine , nuclear medicine , cell , surgery , transplantation , biochemistry , enzyme
Abstract Background Interstitial laser coagulation (ILC) is a method of local tissue destruction for solid tumors such as irresectable hepatic metastases from colorectal cancer. With the availability of new magnetic resonance (MR) techniques, which allow real time tissue temperature mapping, it is essential to know the critical temperature and exposure times leading to cell death. Materials and Methods/Study Design Samples (8 mm 3 ) of solid rat tumor (CC‐531, syngenic to the WAG/Rij rat strain), were warmed in tubes for four different temperatures (40, 50, 60 or 80°C) and four different exposure times (3, 6, 12, or 24 minutes). Combinations were replicated in five‐fold. Cell viability was assessed with three methods: Trypan blue exclusion test in collagenase/dispase dissociated samples, NADH activity in snap frozen samples and outgrowth for 2 weeks under the renal capsule of WAG/Rij rats. Results Results of the three methods revealed that viability was not affected with heating at 40 and 50°C except for 24 minutes at 50°C. At higher temperatures cell death occurred at all exposure times. Conclusion The temperature range resulting in sufficient tissue coagulation for cell death is between 50°C and 60°C for a short duration (<3 minutes). These data can be used to achieve complete tumor destruction and minimal surrounding tissue damage during real‐time MR‐controlled ILC. Lasers Surg. Med. 25:257–262, 1999. © 1999 Wiley‐Liss, Inc.