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Detection of transplant vasculopathy in a rat aortic allograft model by fluorescence spectroscopic optical analysis
Author(s) -
Christov Alexander,
Dai Erbin,
Liu Liying,
Miller Leslie W.,
Nash Piers,
Lalani Alshad,
McFadden Grant,
Nation Patric N.,
Tulip John,
Lucas Alexandra
Publication year - 1999
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1999)24:5<346::aid-lsm5>3.0.co;2-k
Subject(s) - fluorescence , pathology , medicine , chemistry , optics , physics
Background and Objective Transplant vasculopathy is a leading cause of late cardiac graft loss. We have examined laser‐induced fluorescence (LIF) spectroscopy as an optical diagnostic tool for detection of intimal plaque development and inflammatory cellular invasion in a rat model of aortic allograft transplant. Study Design/Materials and Methods Infrarenal aortic segments were transplanted from Lewis to Sprague Dawley rats. A range of vasculopathy development was produced by treatment with a viral anti‐inflammatory protein. LIF spectra were recorded from the intima of aortic implants at 28 days. Fluorescence intensity was analyzed for correlation with vasculopathy development. Results Significant differences in LIF intensity at 400–450 nm ( P ≤ 0.05 by ANOVA) were detected. LIF emission was correlated with plaque growth (R 2 = 0.980), vessel narrowing (R 2 = 0.964), and cellular invasion (R 2 = 0.971) by regression analysis. Conclusion LIF optical analysis provides a nontraumatic diagnostic approach for detection of atherosclerosis prior to cardiac transplant or during development of vasculopathy after transplant. Lasers Surg. Med. 24:346–359, 1999. © 1999 Wiley‐Liss, Inc.