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Effect of low intensity monochromatic light therapy (890 nm) on a radiation‐impaired, wound‐healing model in murine skin
Author(s) -
Lowe Andrea S.,
Walker Mark D.,
O'Byrne Miriam,
Baxter G. David,
Hirst David G.
Publication year - 1998
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1998)23:5<291::aid-lsm9>3.0.co;2-p
Subject(s) - dorsum , wound healing , medicine , irradiation , nuclear medicine , analysis of variance , monochromatic color , surgery , anatomy , biology , botany , physics , nuclear physics
Background and Objective The use of low intensity laser and monochromatic light diodes as a therapeutic modality has become popular in a variety of clinical applications, including the promotion of wound repair. Despite this, the clinical evidence base for such application remains sparse; in contrast, recent studies have demonstrated a number of quantifiable photobiological effects associated with such therapy. In the present study, the effect of low intensity monochromatic light irradiation (MLI) at various radiant exposures upon a radiation‐impaired wound model in murine skin was investigated. Study Design/Materials and Methods Male Balb/c mice (n = 50; age matched at 10 weeks) were randomly allocated to five experimental groups (n = 10 each group). In Group 1, mice were left untreated; in Groups 2–5, a well‐defined area on the dorsum was exposed to 20 Gy X‐ray irradiation. At 72 hours post‐irradiation, all mice were anaesthetised and a 7‐mm‐square area wound was made on the dorsum. All wounds were videotaped alongside a marker scale until closure was complete. In Groups 3–5, mice were treated with MLI (0.18, 0.54, and 1.45 J/cm 2 , respectively) three times weekly using a GaAlAs 890 nm multidiode (n = 60) array unit (270 Hz; maximum rated output, 300 mW; Anodyne, Denver, CO). Subsequently, the area of each wound was measured from video using an image analysis system (Fenestra 2.1), and results were analysed using repeated measure and one‐factor ANOVA statistical tests. Results X‐ray irradiation caused a significant delay ( P = 0.0122) in healing by day 7. MLI at 0.18 J/cm 2 and 0.54 J/cm 2 had no effect upon the rate of wound closure. However, a highly significant ( P = 0.0001) inhibition occurred following MLI irradiation at 1.45 J/cm 2 by day 16. Conclusion These findings provide little evidence of the putative stimulatory effects of monochromatic light irradiation in vivo, but, rather, reveal the potential for an inhibitory effect at higher radiant exposures. Lasers Surg. Med. 23:291–298, 1998. © 1998 Wiley‐Liss, Inc.