Premium
Subcellular phototoxicity of 5‐aminolaevulinic acid (ALA)
Author(s) -
Liang Hong,
Shin David S.,
Lee Yider Eddie,
Nguyen Duc Chi,
Trang Tina Ching,
Pan Ann Huang,
Huang Scott LiJu,
Chong David Huber,
Berns Michael W.
Publication year - 1998
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1998)22:1<14::aid-lsm6>3.0.co;2-#
Subject(s) - phototoxicity , cytoplasm , subcellular localization , chemistry , fluorescence microscope , cell , cell nucleus , photosensitizer , microbiology and biotechnology , fluorescence , biology , biochemistry , in vitro , photochemistry , optics , physics
Background and Objective 5‐aminolaevulinic acid (ALA) is a new, promising photosensitizer for PDT of cancer. Subcellular toxicity induced by ALA and light exposure in single cells was studied to elucidate the mechanism of cell damage. Study Design/Materials and Methods CPAE, PTK 2 , and rat neonatal myocardial cells treated with ALA were examined for localization using fluorescence microscopy and for subcellular phototoxicity using 630 nm laser microbeam irradiation of specific subcellular regions. Results In CPAE and PTK 2 cells, a large amount of fluorescence was detected in the peri‐nuclear cytoplasm. In rat neonatal myocardial cells, the sensitizer selectively localized in the large mitochondria. In both cell types, there was little phototoxicity when the peripheral cytoplasmic region was exposed, as compared to considerable phototoxicity with exposure of either the perinuclear or nuclear regions. Conclusion Both the CPAE and PTK 2 cells demonstrated that the nucleus followed by the perinuclear cytoplasm are the most sensitive cell areas with no sensitivity in the peripheral cytoplasm. Lasers Surg. Med. 22:14–24, 1998. © 1998 Wiley‐Liss, Inc.