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Breast cancer diagnosis using N 2 laser excited autofluorescence spectroscopy
Author(s) -
Gupta Pradeep Kumar,
Majumder Shovan Kumar,
Uppal Abha
Publication year - 1997
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1997)21:5<417::aid-lsm2>3.0.co;2-t
Subject(s) - autofluorescence , breast cancer , pathology , intensity (physics) , medicine , cancer , breast tissue , laser , fluorescence , chemistry , optics , physics
Background and Objective This article reports results of an in vitro study involving 63 patients for the evaluation of the diagnostic potential of N 2 laser excited autofluorescence spectroscopy of human breast tissues. Materials and Methods The N 2 laser‐excited spectra were recorded from benign (fibroadenomas, 35 patients), cancerous (ductal carcinomas, 28 patients), and normal (the uninvolved areas of the resected cancerous specimens). A stepwise multivariate linear regression (MVLR) analysis was developed to analyze the diagnostic content of the breast tissue fluorescence spectra. Results Significant changes were observed in the autofluorescence from normal, benign, and cancerous breast tissues, particularly in the spectrally integrated fluorescence intensity. The ratio of mean spectrally integrated intensity from cancerous tissues to that from benign tumor and normal tissues were 3.2 and 2.8, respectively. A discrimination parameter based on spectrally integrated intensity alone provided a sensitivity and specificity of up to 99.6% over the sample size investigated for discrimination of cancerous breast tissues from benign/normal. Conclusion Our results suggest that a straightforward measurement of the total integrated fluorescence intensity can provide excellent discrimination between cancerous and benign/normal breast tissues. Lasers Surg. Med. 21:417–422, 1997. © 1997 Wiley‐Liss, Inc.