Premium
Helium‐neon laser irradiation is not a stressful treatment: A study on heat‐shock protein (HSP70) level
Author(s) -
Abou Hashieh Imad,
Tardieu Corinne,
Franquin JeanClaude
Publication year - 1997
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1997)20:4<451::aid-lsm12>3.0.co;2-e
Subject(s) - hsp70 , heat shock protein , irradiation , shock (circulatory) , laser , blot , neon , microbiology and biotechnology , mucositis , gel electrophoresis , chemistry , materials science , medicine , chemotherapy , biology , biochemistry , argon , surgery , optics , physics , organic chemistry , nuclear physics , gene
Background and Objective Helium‐neon (He‐Ne) laser irradiation has been clinically used to reduce chemotherapy‐induced mucositis. This work was designed to find out if this treatment is stressful at the cellular level by studying its effects on the level of the stress‐inducible heat shock proteins. Study Design/Materials and Methods: Human desmodontal and mouse L929 fibroblasts were irradiated using a 60 mW laser by a single application of 1.5 and 3J/cm 2 in continuous mode. Heat shock protein level was studied by gel electrophoresis and Western blotting using monoclonal antibodies. Results He‐Ne laser treatment does not induce heat shock protein synthesis in human desmodontal nor in mouse fibroblasts at the energy densities used in this study. Conclusion These results indicate that the treatment is not stressful at the cellular level. Lasers Surg. Med. 20:451–460, 1997. © 1997 Wiley‐Liss, Inc.