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Low energy laser irradiation fails to modulate the inflammatory function of human monocytes and endothelial cells
Author(s) -
Bouma Maarten G.,
Buurman Wim A.,
van den Wildenberg Frans A.J.M.
Publication year - 1996
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1996)19:2<207::aid-lsm13>3.0.co;2-3
Subject(s) - umbilical vein , proinflammatory cytokine , tumor necrosis factor alpha , cytokine , stimulation , cell adhesion molecule , endothelial stem cell , inflammation , downregulation and upregulation , immunology , monocyte , microbiology and biotechnology , chemistry , medicine , in vitro , endocrinology , biology , biochemistry , gene
Background and Objective In view of the important regulatory role of cytokines in wound healing and inflammation, we investigated the effects of low energy laser irradiation on cytokine release by human peripheral blood monocytes (MΦ) and human umbilical vein endothelial cells (HUVEC) in vitro. Also, the effects of laser light on the expression of endothelial adhesion molecules, another important feature of inflammatory and regenerative responses, were assessed. Study Design/Materials and Methods Cells were irradiated with a pulsed GaAs‐laser (904 nm) at energy densities 0 (= sham), 0.3, 3.0, or 9.0 J/cm 2 and subsequently incubated in absence or presence of endotoxin (MΦ) or the proinflammatory cytokines TNFα and IL‐1β (HUVEC). Results Irradiation at any of the dosages used did not significantly affect spontaneous or endotoxin‐induced release of TNFα, IL‐6, and IL‐8 by MΦ. Similarly, secretion of IL‐6 and IL‐8 by resting or cytokine‐activated HUVEC after either single or repeated laser treatment was unchanged as compared to sham‐irradiated controls. Moreover, laser treatment did not induce de novo expression or upregulation of the endothelial adhesion molecules E‐selectin, ICAM‐1, and VCAM‐1, and it failed to modify their expression in response to stimulation with TNFα or IL‐1β. Conclusions We conclude that with the specific laser parameters and dose‐regimen used, low energy laserlight does not affect the inflammatory function of human monocytes and endothelial cells in vitro. © 1996 Wiley‐Liss, Inc.