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Reactive oxygen species inducible by low‐intensity laser irradiation alter DNA synthesis in the haemopoietic cell line U937
Author(s) -
Callaghan Gary A.,
Riordan Cathal,
Gilmore William S.,
McIntyre Irene A.,
Allen James M.,
Hannigan Bernadette M.
Publication year - 1996
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1996)19:2<201::aid-lsm12>3.0.co;2-9
Subject(s) - reactive oxygen species , u937 cell , catalase , dna damage , irradiation , cell culture , dna synthesis , microbiology and biotechnology , thymidine , dna , cell growth , biophysics , chemistry , biology , antioxidant , biochemistry , genetics , physics , nuclear physics
Background and Objective The purpose of this study was to evaluate the possible role of reactive oxygen species (ROS) in mediating previously recorded alterations in DNA synthesis, inducible by low‐intensity laser irradiation (LILI), in the haemopoietic cell line U937. Study Design/Materials and Methods The ability of LILI (660 nm, 12 mW, 5 kHz) to induce ROS from U937 cells was assessed spectrophotometrically at energy densities (E.D.) from 1.0 to 11.5 J/cm 2 . In order to assess whether laser‐induced ROS could alter cellular proliferation DNA synthesis was measured post‐irradiation, by the incorporation of tritiated thymidine ( 3 H‐TdR) into the cells in both the presence and absence of the antioxidant catalase (CAT). Results Detectable ROS were produced posy‐irradiation only from the differentiated form of the cell line. Analysts by Student's t ‐test for unrelated groups showed a significant difference, at E.D.s 2.9 and 8.6 J/cm 2 , in the extent of DNA synthesis occurring in cells irradiated in the presence of CAT or in its absence. Conclusion These findings demonstrate that laser‐inducible ROS can mediate laser's effects on this cell line. © 1996 Wiley‐Liss, Inc.