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Human albumin solders for clinical application during laser tissue welding
Author(s) -
Poppas Dix P.,
Wright E. James,
Guthrie Paul D.,
Shlahet Larisa T.,
Retik Alan B.
Publication year - 1996
Publication title -
lasers in surgery and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 112
eISSN - 1096-9101
pISSN - 0196-8092
DOI - 10.1002/(sici)1096-9101(1996)19:1<2::aid-lsm1>3.0.co;2-w
Subject(s) - human albumin , welding , laser , albumin , pathology , biomedical engineering , materials science , medicine , metallurgy , optics , physics
Background and Objective Fifty percent human albumin solder significantly improves weld strength when compared to lower concentrations [Wright et al., ASLMS meeting, April, 1995]. We developed a method for preparing 50% human albumin that may be considered compatible for clinical applications. Study Design/Materials and Methods Fifty percent human albumin solder was prepared from 25% commercially available human albumin using a lyophilization technique. Assessment of sterility, viscosity, pH, and peak absorption wavelength were performed. Results This report describes the metrology used to prepare a 50% human albumin solder that is compatible with clinical use. Maintenance of the structural integrity of the albumin was confirmed by polyacrylamide gel electrophoresis. Conclusion This solder preparation can be used alone or with the addition of exogenous chromophores. The final product is sterile, incorporates viral free protocols, maintains high viscosity, and can be applied easily during open or laparoscopic procedures. © 1996 Wiley‐Liss, Inc.