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Expression of matrix metalloproteinases and the tissue inhibitors of metalloproteinases and their local invasiveness and metastasis in Chinese human pancreatic cancer
Author(s) -
Gong Y.L.,
Xu G.M.,
Huang W.D.,
Chen L.B.
Publication year - 2000
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(200002)73:2<95::aid-jso7>3.0.co;2-r
Subject(s) - timp1 , mmp2 , matrix metalloproteinase , mmp9 , pathology , medicine , metastasis , pancreatic cancer , pancreas , cancer research , matrilysin , cancer , gene expression , biology , gene , downregulation and upregulation , biochemistry
Background and Objectives The objective was to evaluate the potent role of matrix metalloproteinases(MMPs) and the tissue inhibitors of metalloproteinases(TIMPs) in processes leading to metastasis and local invasiveness of Chinese human ductal adenocarcinomas of the pancreas. We also evaluated a possible biological association between the gene expression and clinical manifestations. Methods Northern blot and in situ hybridization have shown MMP and TIMP gene expression in the pancreas and alterations associated with neoplastic transformation. Fifteen cases of surgical pancreatic specimens were examined, using cDNA probes to MMP2, MMP9, and TIMP1. Findings were correlated with the size of tumor section, CA19‐9, pathological classification, thrombosis, and infiltration of capsule and lymphonoids. Results Increased levels of the mRNA of MMP2, MMP9, and TIMP1 genes, MMP2≈MMP9