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Identification of semaphorin E gene expression in metastatic human lung adenocarcinoma cells by mRNA differential display
Author(s) -
MartínSatué Mireia,
Blanco Jerónimo
Publication year - 1999
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199909)72:1<18::aid-jso5>3.0.co;2-p
Subject(s) - semaphorin , medicine , adenocarcinoma , messenger rna , differential display , identification (biology) , gene expression , gene , lung , metastasis , differential diagnosis , cancer research , pathology , cancer , biology , receptor , genetics , botany
Synopsis Human lung adenocarcinoma cell lines HAL‐8Luc and HAL‐24Luc differ in their metastatic potential. HAL‐8Luc cells metastasize to lungs when injected either intravenously or intramuscularly. in mice while HAL‐24Luc cells do not. The differential display method is used to identify genes differentially expressed between the two cell lines and the findings are extensively discussed. Background Lung cancer is the leading form of cancer in most countries, and metastasis is the main cause of death in oncological patients. The metastatic phenotype of tumor cells is the result of genetic events altering the RNA and protein expression of normal cells. Our objective was to identify genes expressed differentially between metastatic and nonmetastatic human lung adenocarcinoma cells that might be used as a prognostic factor. Methods The differential display technique was used to compare the RNA expression patterns distinguishing metastatic (HAL‐8Luc) and nonmetastatic (HAL‐24Luc) human lung adenocarcinoma cells, two genetically close cell lines. Results Differential expression of three cDNAs was confirmed by Northern blot analysis. Two sequences corresponding to a putative splicing factor and a proliferation‐related factor cDNAs were underexpressed in the metastatic cells relative to the nonmetastatic ones. Interestingly, we found that human semaphorin E mRNA was several fold overexpressed in the metastatic cells. This recently identified gene encodes a protein whose expression has been related to several cell survival mechanisms as well as to immunosuppression. Conclusion Our results point to the relevance of semaphorin E in metastatic spread of human lung adenocarcinoma cells. J. Surg. Oncol. 1999;72:18–23. © 1999 Wiley‐Liss, Inc.