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Induction of human leukocyte antigen (HLA)‐A2‐restricted and MAGE‐3‐gene‐derived peptide‐specific cytolytic T lymphocytes using cultured dendritic cells from an HLA‐A2 esophageal cancer patient
Author(s) -
Kanaoka Shunji,
Yamasaki Seiji,
Okino Takashi,
Inoue Naoya,
Shimada Yutaka,
Kaneko Midori,
Otaka Akira,
Fujii Nobutaka,
Imamura Masayuki
Publication year - 1999
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199905)71:1<16::aid-jso4>3.0.co;2-a
Subject(s) - cytolysis , human leukocyte antigen , immunology , medicine , antigen , gene , cytotoxic t cell , biology , in vitro , genetics
Background and Objectives Using peripheral blood mononuclear cells (PBMCs) from a 10‐year survivor with established human leukocyte antigen (HLA)‐A2(+) and MAGE‐3(+) esophageal cancer cell line (KYSE‐170), we examined the induction of HLA‐A2‐restricted and MAGE‐3‐gene‐derived peptide (FLWGPRALV, amino acids 271–279)‐specific cytolytic T lymphocytes (CTLs). Methods Autologous dendritic cells (DCs) cultured with granulocyte‐macrophage colony stimulating factor and interleukin‐4 were used as antigen presenting cells. PBMCs were stimulated by peptide‐pulsed DCs in vitro. Results PBMC cocultured with FLWGPRALV‐pulsed DCs could induce the relevant peptide‐specific CTLs, which had tumor necrosis factor production and specific cytotoxicity against relevant peptide‐pulsed autologous DCs (34%, effector:target ratio = 40:1). Moreover, they showed specific cytotoxicity against the autologous esophageal cancer cell line KYSE‐170 (17%, effector:target ratio = 40:1). Conclusions These results suggest that FLWGPRALV‐pulsed cultured DCs would be a potent candidate for peptide vaccine against HLA‐A2(+) and MAGE‐3(+) esophageal cancer. J. Surg. Oncol. 1999;71:16–21. © 1999 Wiley‐Liss, Inc.