Premium
Enhanced tumor targeting by an intratumoral injection of colloidal chromic 32 P in two human tumors (AsPC‐1 pancreas and Ls174T colon) in nude mice
Author(s) -
Lee Intae
Publication year - 1999
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199903)70:3<161::aid-jso3>3.0.co;2-s
Subject(s) - medicine , laser doppler velocimetry , pancreas , nuclear medicine , cancer research , pathology , urology , blood flow
Background and Objectives To find the mechanisms of the ongoing clinical trials in intralesional colloidal chromic 32 P ( 32 P‐CP) brachytherapy, the cellular uptake of 32 P‐CP, changes in tumor interstitial fluid pressure (TIFP), and tumor blood flow (TBF) using two (AsPC‐1, Ls174T) human tumors were measured. Methods After exposure to 32 P‐CP using exponential and plateau‐phase cells, cells were trypsinized at various time intervals, then measured for the levels of radioactivity using a γ‐counter. Also measured were TIFP using the WIN technique and TBF with laser Doppler flowmetry. Results The plateau growth‐phase of both tumors showed the maximal uptake of 32 P‐CP at ∼100 min. TBF decreased within 10 min after an intratumoral (i.t.) injection of 32 P‐CP, and reached 75% of control value by 1 h. Conclusions If 32 P‐CP was introduced i.t., it maintained highly efficient tumor targeting, mainly due to two physiological mechanisms: the high adherence of 32 P‐CP to the infused regions and the reduction in TBF by this therapeutic colloid. J. Surg. Oncol. 1999;70:161–166. © 1999 Wiley‐Liss, Inc.