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Expression of insulin‐like growth factor receptor, IGF‐1, and IGF‐2 in primary and metastatic osteosarcoma
Author(s) -
Burrow Sarah,
Andrulis Irene L.,
Pollak Michael,
Bell Robert S.
Publication year - 1998
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199809)69:1<21::aid-jso5>3.0.co;2-m
Subject(s) - osteosarcoma , autocrine signalling , insulin like growth factor , medicine , growth factor , receptor , insulin like growth factor 2 , cancer research , stimulation , cell culture , endocrinology , biology , genetics
Background and Objectives: We have previously shown that insulin‐like growth factor (IGF)‐responsive murine sarcomas demonstrate inhibition of local and metastatic disease growth when implanted in an IGF‐deficient host animal. In this experiment, we tested whether IGF receptor (IGF‐R) and ligands were expressed in human primary and metastatic osteosarcomas. Methods Fifty‐two specimens of human osteosarcoma tumor from 48 patients were assayed for IGF‐R, IGF‐1, and IGF‐2 using reverse transcriptase polymerase chain reaction. Results Twenty‐one of 46 tumors analyzed had levels of expression of IGF‐R greater than or equal to the positive control cell line. Twenty‐seven of 44 expressed levels of IGF‐1 greater than or equal to the positive control, as did 21 of 38 cases assayed for IGF‐2. No differences were found between 40 primary tumor samples and 12 metastatic lesions in mean levels of IGF‐R, IGF‐1, or IGF‐2. There was a moderately strong correlation between expression of IGF‐R and IGF‐1, suggesting that autocrine stimulation may be an important mechanism for stimulation of osteosarcoma proliferation. Conclusions A significant proportion of osteosarcoma tumors express IGF‐R and ligands. Higher levels of expression were not correlated with metastatic lesions. J. Surg. Oncol. 1998;69:21–27. © 1998 Wiley‐Liss, Inc.