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Altered composition and increased endothelial cell proliferative activity of proteoglycans isolated from breast carcinoma
Author(s) -
Vijayagopal Parakat,
Figueroa Julio E.,
Levine Edward A.
Publication year - 1998
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199808)68:4<250::aid-jso9>3.0.co;2-3
Subject(s) - proteoglycan , extracellular matrix , chondroitin sulfate proteoglycan , dermatan sulfate , cell growth , chondroitin sulfate , endothelial stem cell , breast carcinoma , glycosaminoglycan , chemistry , biochemistry , medicine , breast cancer , cancer , in vitro
Background and Objectives: Proteoglycans of the extracellular matrix are vital to the growth and evolution of malignant neoplasms. The present study determined the composition of proteoglycans isolated from paired specimens of normal breast and adenocarcinoma of the breast harvested from each patient (n = 8). The proteoglycans were then tested for their ability to stimulate endothelial cell proliferation. Methods Proteoglycans were isolated by extraction with 4M guanidine hydrochloride and purified by CsCl density‐gradient centrifugation. The proteoglycans were characterized and tested for their ability to simulate endothelial cell proliferation. Results In each case, the total proteoglycan content of the tumor was significantly greater than that of the corresponding normal tissue. The proteoglycans isolated from the carcinoma contained 32.2% (13.7/42.5) more chondroitin sulfate, 18.5% (5.6/30.2) less dermatan sulfate, and 29.6% (8.1/27.3) less heparan sulfate than did the proteoglycans of normal breast tissue. Proteoglycans from normal tissue did not stimulate endothelial cell proliferation, whereas those from malignant tissue stimulated proliferation by 1.3‐ to 1.5‐fold. Conclusions These results indicate that malignant breast tissue exhibits both qualitative and quantitative changes in proteoglycan composition, which, in turn, may stimulate endothelial cell proliferation. J. Surg. Oncol. 68:250–254, 1998 . © 1998 Wiley‐Liss, Inc.