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Haptotactic migration of pancreatic cancer cells induced by bioactive components in bovine liver extract
Author(s) -
Ito Hiroshi,
Miyazaki Masaru,
Nishimura Fusanori,
Nakajima Nobuyuki
Publication year - 1998
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199807)68:3<153::aid-jso4>3.0.co;2-4
Subject(s) - medicine , pancreatic cancer , liver cancer , cancer , cancer research , pathology
Background and Objectives: Migration into subendothelial tissue in remote organ by cancer cells are crucial events for organ‐specific metastasis, including liver metastasis. This study aims to investigate the chemoattractive ingredients in liver extract, which induce migration of liver metastatic cancer cells. Methods Cell migrations of SU.86.86 cells, human pancreatic cancer cells raised from liver metastasis, toward the bovine liver extract were studied by chemotaxis or haptotaxis assay. Bovine liver extract was partially purified by chromatographic or gel elutriation method. Results The soluble fraction of the liver extract did not induce chemotactic migration of SU.86.86 cells. However, the insoluble fraction induced a remarkable haptotactic migratory response. C 18 column unbound insoluble fractions eluted from SDS‐PAGE induced chemotactic migration of SU.86.86 cells. Conclusion Such bioactive components in liver extract may play an important role in the development of liver metastasis. J. Surg. Oncol. 1998;68:153–158. © 1998 Wiley‐Liss, Inc.