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Application of the differential hybridization of Atlas™ human expression arrays technique in the identification of differentially expressed genes in human glioblastoma multiforme tumor tissue
Author(s) -
Sehgal Anil,
Boynton Alton L.,
Young Ronald F.,
Vermeulen Sandra S.,
Yonemura Kenneth S.,
Kohler Erik P.,
Aldape Hector C.,
Simrell Charles R.,
Murphy Gerald P.
Publication year - 1998
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199804)67:4<234::aid-jso5>3.0.co;2-9
Subject(s) - complementary dna , gene , gene expression , microbiology and biotechnology , biology , polymerase chain reaction , glioblastoma , retinoblastoma , reverse transcription polymerase chain reaction , in situ hybridization , differential display , gene expression profiling , genetics , cancer research
Background and Objectives: Several molecular biology techniques are utilized to study changes in gene expression during the genesis of human tumors. Our objective was to identify genes that showed altered expression between normal brain tissue (NBT) and glioblastoma multiforme tumor tissue (GMTT). Methods The technique of differential hybridization of two Atlas™ Human cDNA expression array was used. In this technique, dCTP 32 ‐labeled complimentary DNA from NBT and GMTT was hybridized to two identical human cDNA expression array membranes containing 588 known genes. Results Autoradiographic analysis showed that of the 588 genes analyzed, 52 are overexpressed in GMTT and 57 in NBT. A gene‐specific semiquantitative reverse transcription polymerase chain reaction (RT‐PCR) method was used to confirm the expression pattern of seven known genes. RT‐PCR results demonstrate that the expression pattern of a majority of genes agreed with the expression pattern observed on expression array. The known tumor suppressor genes retinoblastoma (RB) and p53 showed loss of expression in GMTT compared with NBT. Conclusions We conclude that the differential hybridization technique of Atlas™ Human cDNA expression array can be a useful method in identifying genes that are differentially expressed either in NBT or GMTT. J. Surg. Oncol. 1998;67:234–241. © 1998 Wiley‐Liss, Inc.