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The role of stromal cells in the expression of interstitial collagenase (matrix metalloproteinase‐1) in the invasion of gastric cancer
Author(s) -
Sakurai Yoshihiko,
Otani Yoshihide,
Kameyama Kaori,
Igarashi Naoki,
Kubota Tetsuro,
Kumai Koichiro,
Kitajima Masaki
Publication year - 1997
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199711)66:3<168::aid-jso3>3.0.co;2-a
Subject(s) - interstitial collagenase , collagenase , matrix metalloproteinase , stromal cell , medicine , metalloproteinase , cancer , cancer research , pathology , matrix metalloproteinase 3 , enzyme , biology , biochemistry
Background and Objectives In one of the steps of tumor invasion and metastasis, tumor cells must invade surrounding tissues and degrade the components of the basement membranes. Interstitial collagenase (matrix metalloproteinase‐1: MMP‐1) has been also investigated in relation to cancer invasion and metastasis. Methods We investigated the formation and mechanism of MMP‐1 expression using gastric cancer cell lines and gastric fibroblasts derived from normal gastric mucosa by ELISA and immunohistochemistry. Results Production of MMP‐1 protein in gastric fibroblasts was stimulated significantly by adding the conditioned medium of MKN‐74. Localization of MMP‐1 protein in the xenografted gastric cancer cell lines was heterogeneous according to different cell lines. Conclusions These results suggested that the production of MMP‐1 protein in tumor invasion was regulated by interaction between stromal cells, particularly fibroblasts, and tumor cells. J. Surg. Oncol. 1997;66:168–172. © 1997 Wiley‐Liss, Inc.