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Interlaboratory variation in oxygen tension measurement by Eppendorf “Histograph” and comparison with hypoxic marker
Author(s) -
Nozue Mutsumi,
Lee Intae,
Yuan Fan,
Teicher Beverly A.,
Brizel David M.,
Dewhirst Mark W.,
Milross Christopher G.,
Milas Luka,
Song Chang W.,
Thomas Carole D.,
Guichard Marcelle,
Evans Sydney M.,
Koch Cameron J.,
Lord Edith M.,
Jain Rakesh K.,
Suit Herman D.
Publication year - 1997
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199709)66:1<30::aid-jso7>3.0.co;2-o
Subject(s) - medicine , oxygen tension , fibrosarcoma , pathology , immunohistochemistry , staining , hypoxia (environmental) , oxygen , nuclear medicine , andrology , chemistry , organic chemistry
Background and Objectives The median of pO 2 values in tumor measured by Eppendorf “Histograph” with a needle‐type electrode has been used as a prognostic indicator in cancer patients. However, it is not established that a pretreatment measured pO 2 value can be used as a universal predictor of local control probability, because the variation in pO 2 values, especially in hypoxic tissue, among institutes may not allow comparison of measured “absolute pO 2 values.” The purpose of this study was to examine the variation in oxygen tension measurement by Eppendorf “Histograph” among six laboratories using a single batch of mice and tumors and the same detailed protocol. These results were also compared to the immunohistochemical staining of 2‐nitroimidazole adducts. Methods C3H mice bearing FSaII murine fibrosarcoma subcutaneously were shipped to all laboratories, and the oxygen status in tumors and in normal subcutis was examined using Eppendorf “Histograph” and immunohistochemical hypoxic marker. Results All laboratories showed that the FSaII tumor was hypoxic with at least 77% of measured points under 10 mmHg in pO 2 and with a median pO 2 value less than that of normal subcutis. These results were further confirmed immunohistochemically. These findings are interpreted as evidence that the pO 2 values measured by Eppendorf “Histograph” can be useful. However, the median values 5of tumor pO 2 varied from 1.5 mmHg to 5.6 mmHg among the laboratories, and pO 2 of normal subcutis also varied from 28 mmHg to 38 mmHg. There were also significant differences in hypoxic fraction, defined as the fraction under a given oxygen partial pressure (i.e., under 2.5, 5, or 10 mmHg), among institutes. Conclusions Caution needs to be exercised in using the absolute, median, or distribution of pO 2 values measured by the Eppendorf “Histograph” to compare the data between laboratories or to predict the radiation response in an individual subject. J. Surg. Oncol. 1997;66:30–38. © 1997 Wiley‐Liss, Inc.