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Effect of preoperative 5‐fluorouracil on apoptosis of advanced gastric cancer
Author(s) -
Inada Takao,
Ichikawa Akira,
Igarashi Seiji,
Kubota Tetsuro,
Ogata Yoshiro
Publication year - 1997
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199706)65:2<106::aid-jso6>3.0.co;2-b
Subject(s) - apoptosis , medicine , oncogene , chemotherapy , cancer , fluorouracil , cancer research , cell cycle , pathology , biology , biochemistry
Background Several studies have reported on apoptosis and the effect of anticancer chemotherapy. Methods We studied apoptosis induced by 5‐fluorouracil (5‐FU) given preoperatively to 28 patients with advanced gastric cancer and compared the findings with 101 untreated patients. The expression of bcl‐2 oncoprotein, cell phase fractions, and histological chemotherapeutic effects were also compared with the apoptotic changes. Results The apoptotic and S‐phase fractions in 5‐FU‐treated patients (apoptotic fraction: 10.46 ± 6.93%, S‐phase fraction: 17.49 ± 11.65%) were significantly greater than those in untreated controls (apoptotic fraction: 6.56 ± 5.06%, S‐phase fraction: 12.17 ± 6.78%). A positive correlation was observed between 5‐FU‐induced apoptosis and accumulation of tumor cells in the S‐phase fraction. There was an inverse relationship between bcl‐2 oncoprotein expression and apoptosis in 5‐FU‐treated patients, but no significant correlation between histological effect and apoptosis. However, two patients with significant histological effects showed no bcl‐2 oncoprotein expression, whereas the histological effects were mild in all the bcl‐2 ‐positive patients. Conclusions Apoptosis may be induced by 5‐FU administered preoperatively and bcl‐2 oncogene expression may suppress 5‐FU‐induced apoptosis. J. Surg. Oncol. 1997;65:106–110. © 1997 Wiley‐Liss, Inc.