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Granulocyte colony‐stimulating factor and interleukin‐6‐producing lung cancer cell line, LCAM
Author(s) -
Inoue Masayoshi,
Minami Masato,
Fujii Yoshitaka,
Matsuda Hikaru,
Shirakura Ryota,
Kido Tetsuo
Publication year - 1997
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199704)64:4<347::aid-jso18>3.0.co;2-#
Subject(s) - medicine , granulocyte colony stimulating factor , granulocyte , lung cancer , interleukin 2 , interleukin , cancer research , oncology , immunology , cytokine , chemotherapy
Background and Objectives: We describe a case of granulocyte colony‐stimulating factor (GCSF) and interleukin‐6 (IL‐6)‐producing lung cancer. Methods A 53‐year‐old man underwent left upper lobectomy under diagnosis of lung cancer. The tumor obtained by a preoperative biopsy was analyzed. Results Preoperative data showed leukocytosis with left‐shift of leukocytic morphology and thrombocytosis and an elevated serum GCSF level. Histological examination revealed poorly differentiated adenocarcinoma. A cell line, named LCAM, was established from the tumor and the cytokines in the culture medium were measured by enzyme immunoassay. GCSF and IL‐6 were produced in large amounts by LCAM, but granulocyte‐macrophage colony‐stimulating factor (GMCSF) and interleukin‐3 (IL‐3) were not. A proportion of LCAM expressed GCSF receptor on the cell surface, but IL‐6 receptor could not be detected. LCAM proliferation was inhibited in the culture with antihuman GCSF antibody in a dose‐dependent manner. Conclusions We suggest that LCAM proliferation is positively regulated by GCSF. J. Surg. Oncol. 64:347–350, 1997. © 1997 Wiley‐Liss, Inc.