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Melanoma antigen‐encoding gene‐1 expression in invasive gastric carcinoma: Correlation with stage of disease
Author(s) -
Katano Mitsuo,
Nakamura Mitsunari,
Morisaki Takashi,
Fujimoto Kazuma
Publication year - 1997
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199703)64:3<195::aid-jso4>3.0.co;2-5
Subject(s) - gene expression , cancer research , mmp2 , gene , cancer , plasminogen activator , pathology , gene expression profiling , vascular endothelial growth factor , biology , medicine , metastasis , vegf receptors , genetics
Background A human melanoma antigen‐encoding gene‐1, MAGE‐1 gene, may be linked to the neoplastic transformation. In the present study, we extended this association with human gastric carcinomas. Specifically, we focused on the relationship between MAGE‐1 gene expression and the histologic stage of gastric carcinoma. Methods We used a reverse transcription‐polymerase chain reaction assay (RT‐PCR) to analyze the expression of the MAGE‐1 gene in 38 endoscopic biopsy specimens from gastric carcinomas. We also studied the relationship between the expression of MAGE‐1 gene and the genetic expression of several tumor invasion‐related factors, including 72 kD type IV collagenase (MMP2), urokinase‐type plasminogen activator (uPA), platelet‐derived growth factor A (PDGF‐A), and vascular endothelial growth factor (VEGF). Results Eleven of the 38 tumor samples (28.9%) expressed the MAGE‐1 gene. MAGE‐1 gene expression was present only in two of the 38 adjacent nontumor samples (5.3%). MAGE‐1 gene expression in the 38 tumor samples was significantly correlated with the histological stage of disease ( P = 0.0008), especially with the depth of histologically confirmed tumor invasion (tl vs. t2 or greater, P = 0.00048). The expression of MAGE‐1 gene correlated with the expression of MMP2 ( P = 0.0064), uPA ( P = 0.0390) and PDGF‐A ( P = 0.00018). Conclusions These data suggest that the MAGE‐1 gene may be activated in gastric carcinomas during periods of their development or invasion. In addition, a relationship between MAGE‐1 gene expression and expression of invasion‐related factors has been demonstrated. J. Surg. Oncol. 64:195–201, 1997 © 1997 Wiley‐Liss, Inc.