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Immunosuppressive cytokines (IL‐10, TGF‐β) genes expression in human gastric carcinoma tissues
Author(s) -
Morisaki Takashi,
Katano Mitsuo,
Ikubo Akashi,
Anan Keisei,
Nakamura Mitsunari,
Nakamura Kenjiro,
Sato Hiroshi,
Tanaka Masao,
Torisu Motomichi
Publication year - 1996
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199612)63:4<234::aid-jso4>3.0.co;2-d
Subject(s) - transforming growth factor , messenger rna , cancer , medicine , metastasis , cancer research , immunosuppression , tumor progression , pathology , pathological , cytokine , in vivo , biology , gene , immunology , biochemistry , microbiology and biotechnology
Background Contribution of immunosuppressive cytokines to tumor progression in many types of cancers has been suggested. To characterize the in vivo expression of immunosuppressive cytokines in gastric cancer, we analyzed the messenger RNA (mRNA) expression of transforming growth factor‐β (TGF‐β) and interleukin‐10 (IL‐10) in human gastric carcinoma tissues. Methods Both tumor tissues and nontumor tissues from each resected specimen of 29 primary gastric carcinomas were tested for IL‐10 and TGF‐β mRNA expression by the reverse transcriptase‐polymerase chain reaction (RT‐PCR), and the mRNA expression was correlated with various pathological parameters of the tumors. Results Among the 29 tumors, mRNAs of TGF‐β and IL‐10 were detected in 79% and 62% of tumor samples, respectively. These cytokines were detected only in 31% for TGF‐β and 17% for IL‐10 in nontumor samples. Both mRNAs were frequently expressed in the poorly differentiated adenocarcinomas and the tumor tissues with high degree of stage or lymphnode metastasis. Conclusions Local expression of immunosuppressive cytokines may contribute to the progression of primary gastric carcinomas possibly through immunosuppression. © 1996 Wiley‐Liss, Inc.