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Establishment of xenografts and cell lines from well‐differentiated human thyroid carcinoma
Author(s) -
Lin JenDer,
Chao TzuChieh,
Weng HsiaoFen,
Huang HongSo,
Ho YatSen
Publication year - 1996
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199610)63:2<112::aid-jso8>3.0.co;2-h
Subject(s) - thyroid cancer , thyroid , cell culture , cancer , thyroid carcinoma , medicine , cancer cell , pathology , transplantation , cancer research , cell , follicular thyroid cancer , endocrinology , papillary thyroid cancer , biology , biochemistry , genetics
Background Our aim was to establish Chinese human thyroid cancer cell lines and to provide the material for thyroid cancer research. Methods We collected thyroid cancer tissues from 93 patients with thyroid cancer for developing the primary culture. Thyroid cancer tissues were verified by frozen sections during the operations, then subjected to primary cell culture. During the first several passages, fibroblasts were removed by selective attachment. Results From the 93 cancer tissues used, two follicular cancer tissues from the metastatic area (CGTH W‐1, CGTH W‐2) and one papillary thyroid cancer tissue (CGTH W‐3) could be passed over 50 times. Cellular transformation with loss of contact inhibition occurred during passages 5–8. Electron microscopic studies of the CGTH W‐1 cell line showed the presence of an abundance of mitochondria and Golgi complex. Presence of microvilli with interdigitations between neighboring cells were found in CGTH W‐2 and CGTH W‐3 cell lines. Severe combined immunodeficient (SCID) mice were used to determine whether these cells were tumorigenic. Two months after transplantation of 1 × 10 7 CGTH W‐1, 2, and 3 cells into SCID mice, subcutaneous tumors ∼ 2–2.5 cm in size were clearly visible. After affinity cross‐linking, an IGF‐I with insulin‐like growth factor binding proteins complex corresponding to a molecular size of 41 kDa was observed in culture media collected from CGTH W‐1, SW 579, and RO82 W‐1 cells. Conclusions Three thyroid cancer cell lines were established, which may provide material for future thyroid cancer research. © 1996 Wiley‐Liss, Inc.