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Characterization of F3II, a sarcomatoid mammary carcinoma cell line originated from a clonal subpopulation of a mouse adenocarcinoma
Author(s) -
Alonso Daniel F.,
Farías Eduardo F.,
Urtreger Alejandro,
Ladeda Virginia,
Vidal María del Carmen C.,
Joffé Elisa Bal de Kier
Publication year - 1996
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199608)62:4<288::aid-jso14>3.0.co;2-1
Subject(s) - adenocarcinoma , pathology , metastasis , biology , sarcomatoid carcinoma , cell culture , carcinoma , cancer research , mammary gland , mammary tumor , cell , cancer , medicine , breast cancer , genetics
We characterized a new mammary tumor cell line, F3II, previously established in vitro from a clonal subpopulation of the BALB/c transplantable mammary adenocarcinoma M3, moderately metastatic to lung. The F3II cell line has been passaged >50 times. It has grown as elongated cells adherent to the bottom of the flask. Cytogenetic studies showed that F3II cultures were nearly triploid. Tumor cells expressed fibronectin and showed high levels of cell‐surface urokinase, a key protease in invasion and metastasis. F3II cells grew as poorly differentiated, spindle‐cell carcinoma tumors (sarcomatoid carcinomas) with a prominent local invasiveness, a high angiogenic response, and a 90–100% incidence of lung metastases when inoculated s.c. into syngeneic mice. Ultrastructural and immunocytochemical analysis revealed characteristic features of carcinomas. Our data suggest that F3II is less differentiated and more aggressive than the original tumor line, supporting the notion that mammary carcinomas are heterogeneous neoplasms and contain subpopulations with diverse biologic behavior. The F3II mouse mammary sarcomatoid carcinoma line is a suitable model to examine antiinvasive, antiangiogenic, and antimetastatic agents. © 1996 Wiley‐Liss, Inc.