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Expression of MRP and mdr1 in human gastrointestinal cancer cell lines: A correlation with resistance against doxorubicin
Author(s) -
Murase Masaharu,
Kodera Yasuhiro,
Kondo Ken,
Sekiguchi Hiroyuki,
Fujiwara Michitaka,
Kasai Yasushi,
Akiyama Seiji,
Ito Katsuki,
Takagi Hiroshi
Publication year - 1996
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/(sici)1096-9098(199603)61:3<223::aid-jso12>3.0.co;2-8
Subject(s) - doxorubicin , phenotype , multiple drug resistance , anthracycline , gene , cell culture , multidrug resistance associated proteins , gene expression , drug resistance , cancer research , biology , efflux , cancer , medicine , microbiology and biotechnology , chemotherapy , genetics , atp binding cassette transporter , transporter , breast cancer
The mRNA expression of mdr1 and MRP, each of which codes for a transport protein belonging to ATP‐binding cassette superfamily and are reported to be responsible for multidrug resistance phenotype, were semiquantified by RT‐PCR in a panel of gastrointestinal cancer cell lines. Although the expression of MRP was predominant in esophageal cancer cell lines, expression of either or both of the genes was detected in all the cell lines tested. Expression of these two genes added together correlated significantly with chemosensitivity against doxorubicin, implicating that expression of both genes should be evaluated in the future analysis of multidrug resistance phenotype. The ID 50 values for pirarubicin, although generally lower than the values for doxorubicin, correlated well with the latter, suggesting that the similar phenotype as that for doxorubicin might be responsible for drug resistance against this semisynthetic anthracycline glycoside. © 1996 Wiley‐Liss, Inc.