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Characterisation of rotaviruses from children treated at a London hospital during 1996: Emergence of strainsG9P2A[6] and G3P2A[6]
Author(s) -
Cubitt W.D.,
Steele A.D.,
Iturriza M.
Publication year - 2000
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(200005)61:1<150::aid-jmv24>3.0.co;2-w
Subject(s) - serotype , rotavirus , genotype , virology , typing , medicine , pediatrics , polymerase chain reaction , university hospital , biology , virus , microbiology and biotechnology , genetics , gene
Rotavirus strains from 171 patients treated in 1996 at a children's hospital in London were characterised. Use of a panel of typing monoclonal antibodies for serotypes G1–4 identified 105 (61%) of the strains. The majority, 90 strains (86%), were serotype G1. Characterisation of G (VP7) and P (VP4) types using reverse transcription–polymerase chain reaction was more efficient, and 167 of 171 (98%) of the strains were identified this way. The predominant strains were G1P1A[8] (55%) and G4P1A[8] (17%), which are prevalent throughout the world; however, a significant number of cases were associated with genotypes not recorded previously in the United Kingdom. There were 21 (13%) cases associated with G9P2A[6] and 11 (6%) cases associated with G3P2A[6]. The majority (seven of 10) cases of infection in children older than 3 years of age were caused by these two genotypes. A majority (15/21) of G9P2A[6] strains were recovered from children admitted to the hospital, and five children were sufficiently dehydrated to necessitate intravenous rehydration. J. Med. Virol. 61:150–154, 2000. © 2000 Wiley‐Liss, Inc.