Premium
Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of São Paulo, Brazil
Author(s) -
Tanaka Keico,
Itoh Norihiko,
SaitohInagawa Waka,
Uchio Eiichi,
Takeuchi Satoshi,
Aoki Koki,
Soriano Eduardo,
Nishi Mauro,
Júnior Rubens Belfort,
Hársi Charlotte M.,
TsuzukiWang Liping,
Durigon Edison L.,
Stewien Klaus E.,
Ohno Shigeaki
Publication year - 2000
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(200005)61:1<143::aid-jmv23>3.0.co;2-z
Subject(s) - xhoi , hindiii , bglii , virology , restriction enzyme , biology , restriction fragment length polymorphism , genome , bamhi , restriction site , polymerase chain reaction , microbiology and biotechnology , genetics , dna , gene
Genome analysis was carried out on adenovirus strains isolated from patients with acute follicular conjunctivitis in the city of São Paulo, Brazil. Eighteen conjunctival scrapings, collected between December 1993 and March 1994, were analyzed by two methods: a combination of polymerase chain reaction with restriction fragment length polymorphism and viral DNA restriction analysis, carried out using 10 restriction endonucleases: Bam HI, Bgl I, Bgl II, Hin dIII, Kpn I, Sac I, Sal I, Sma I, Xba I, and Xho I. Among 11 adenovirus detected by cell culture isolation, nine were Ad8, and two were Ad7. By restriction analysis the Ad8 isolates were typed as two new variants–Ad8/D11 (seven of nine samples) and Ad8/D12 (two of nine samples). Ad7 isolates were identified as a subtype of the widespread genome type Ad7b and the virulent type Ad7h, a predominant genome type circulating in Argentina, Chile, and Uruguay but absent in Brazil until 1991. J. Med. Virol. 61:143–149, 2000. © 2000 Wiley‐Liss, Inc.