Premium
Reactivation of human herpesvirus 6 by infection of human herpesvirus 7
Author(s) -
TanakaTaya Keiko,
Kondo Toshio,
Nakagawa Naoko,
Inagi Reiko,
Miyoshi Hiroko,
Sunagawa Tomimasa,
Okada Shintaro,
Yamanishi Koichi
Publication year - 2000
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>3.0.co;2-8
Subject(s) - human herpesvirus 6 , virology , peripheral blood mononuclear cell , virus , antigen , herpesviridae , biology , in vitro , human herpesvirus , immunofluorescence , antibody , exanthem , immunology , viral disease , biochemistry
We have attempted to reactivate human herpesvirus 6 (HHV‐6) by infection with HHV‐7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV‐6 and were infected by human herpesvirus 7 (HHV‐7) in vitro. The antigen positive rate to HHV‐6 started to increase 7 days after the infection and reached a maximum by Day 15 using an immunofluorescence antibody test. The copy number of HHV‐6 DNA also increased in the samples in 10 days after infection in vitro. No antigen or increase in DNA was detected in PBMCs, that were mock‐infected or infected with supernatant of stock virus after ultracentrifugation, suggesting that an infection by HHV‐7 is necessary to reactivate HHV‐6. In the paired sera samples during the acute and the convalescent phases of ES, seven to ten bands, that were specific for HHV‐6, were recognized in samples from the acute phase, and at least 5 dominant polypeptides were found more intensively after HHV‐7 infection. J. Med. Virol. 60:284–289, 2000. © 2000 Wiley‐Liss, Inc.