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Expression and antigenicity of human herpesvirus 8 encoded ORF59 protein in AIDS‐associated Kaposi's sarcoma
Author(s) -
Katano Harutaka,
Sata Tetsutaro,
Suda Tetsuji,
Nakamura Tomoko,
Tachikawa Natsuo,
Nishizumi Hirofumi,
Sakurada Shinsaku,
Hayashi Yukiko,
Koike Morio,
Iwamoto Aikichi,
Kurata Takeshi,
Mori Shigeo
Publication year - 1999
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199911)59:3<346::aid-jmv15>3.0.co;2-4
Subject(s) - virology , antigenicity , immunoscreening , biology , antigen , antibody , virus , recombinant dna , in situ hybridization , immunohistochemistry , kaposi's sarcoma associated herpesvirus , microbiology and biotechnology , herpesviridae , complementary dna , cdna library , viral disease , immunology , gene , gene expression , biochemistry
Human herpesvirus 8 (HHV‐8, Kaposi's sarcoma‐associated herpesvirus, KSHV) is a new herpes virus isolated from patients with AIDS‐associated Kaposi's sarcoma (AIDS‐KS). The ORF59 protein of HHV‐8 has recently been shown to encode a processivity factor (PF‐8) for HHV‐8‐encoded DNA polymerase. By immunoscreening a cDNA library derived from the HHV‐8‐infected cell line TY‐1, ORF59 antigen was identified in AIDS‐KS patients. Immunoblotting revealed that recombinant ORF59 protein reacted with sera from patients with AIDS‐KS. Enzyme‐linked immunosorbent assay (ELISA) using ORF59‐recombinant protein as the antigen revealed that 7 of 22 (31.8%) AIDS‐KS patients and 6 of 263 (2.2%) Japanese HIV‐negative patients or healthy blood donors were positive for anti‐ORF59 antibodies. Immunohistochemistry using anti‐ORF59 rabbit antibodies revealed that this protein was expressed in some of the tumor cells found in KS tissues and that ORF59 protein was detected in 11 of 22 (50%) AIDS‐KS tissues. In situ hybridization indicated that some of KS tumor cells were positive for HHV‐8 T1.1 mRNA in the same specimen. These data suggest that ORF59 is one of the HHV‐8 encoded antigens in patients with AIDS‐KS and also indicated that viral replication occurred in some of KS tumor cells. J. Med. Virol. 59:346–355, 1999. © 1999 Wiley‐Liss, Inc.