Premium
Four new genotypes of adenovirus type 3 isolated from patients with conjunctivitis in Japan
Author(s) -
Itoh Norihiko,
Tanaka Keico,
Aoki Koki,
Hinokuma Rikutaro,
Nakagawa Hisashi,
Takeuchi Satoshi,
Uchio Eiichi,
Shiao Shia,
Ohno Shigeaki
Publication year - 1999
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199909)59:1<73::aid-jmv12>3.0.co;2-x
Subject(s) - genotype , serotype , biology , virology , mastadenovirus , restriction enzyme , genome , hindiii , typing , adenoviridae , genetics , gene , recombinant dna
Adenovirus type 3 (Ad3) was the most frequently isolated serotype from patients with conjunctivitis during the period from 1989 to 1991 in Japan. All Ad3 strains isolated in 1990 had an identical genotype, Ad3f. However, in 1992, the predominant serotype was replaced by Ad4. The genome type was examined to determine whether genetic changes existed in Ad3 isolates in 1992. Ad3 isolated from 55 patients with acute conjunctivitis during the period from June 1992 to February 1993 in Japan was assessed by genome typing with restriction endonucleases Bam H I, Bgl II, Hin d III, and Sma I recognizing 6‐base‐pair sequences. The emergence of four new genotypes of Ad3 was identified; one with a new Bam HI site (one isolate), one with a new Bgl II site (two isolates), one with a new Hin d III site (three isolates) and one with a new Sma I site (two isolates). This study demonstrates that the emergence of a new genotype of Ad3 may contribute to the replacement of the predominant serotype associated with adenovirus conjunctivitis in Japan. J. Med. Virol. 59:73–77, 1999. © 1999 Wiley‐Liss, Inc.