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Detection of a novel DNA virus (TTV) sequence in peripheral blood mononuclear cells
Author(s) -
Okamura Akiko,
Yoshioka Mikio,
Kubota Mitsuru,
Kikuta Hideaki,
Ishiko Hiroaki,
Kobayashi Kunihiko
Publication year - 1999
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199906)58:2<174::aid-jmv12>3.0.co;2-x
Subject(s) - virology , peripheral blood mononuclear cell , polymerase chain reaction , biology , virus , dna , nested polymerase chain reaction , dna sequencing , torque teno virus , population , microbiology and biotechnology , gene , medicine , genetics , environmental health , in vitro
DNA sequences of a novel DNA virus (TTV) were examined in 81 peripheral blood mononuclear cell (PBMC) DNA samples from 48 children and 33 adults, 22 cord blood mononuclear cells (CBMC) DNA samples, and 7 autopsy liver tissue DNA samples by a hemi‐nested polymerase chain reaction (PCR). The PCR was carried out using the published primers (NG059, NG061, NG063) to amplify TTV DNA sequences. The sequences were detected in 4 of 81 (5%) PBMC DNA samples, in none of 22 (0%) CBMC DNA samples, and in 2 of 7 (29%) liver tissue DNA samples by direct gel analysis. The PCR‐amplified products were confirmed by direct sequencing. The sequencing showed considerable diversities, with differences of 0–55% in 6 TTV isolates, compared with the prototype sequence of TTV. These results suggest that TTV is a ubiquitous virus that produces asymptomatic infection in a large proportion of the general population without transfusion of blood‐derived products. To our knowledge, this is the first report describing the detection of TTV DNA sequences in PBMCs. J. Med. Virol. 58:174–177, 1999. © 1999 Wiley‐Liss, Inc.