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Induction of intercellular adhesion molecule 1 gene expression by measles virus in human umbilical vein endothelial cells
Author(s) -
Harcourt Brian H.,
Rota Paul A.,
Hummel Kimberly B.,
Bellini William J.,
Offermann Margaret K.
Publication year - 1999
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/(sici)1096-9071(199901)57:1<9::aid-jmv2>3.0.co;2-2
Subject(s) - umbilical vein , virology , measles virus , intercellular adhesion molecule 1 , intracellular , biology , virus , gene , adhesion , morbillivirus , vero cell , cell adhesion molecule , microbiology and biotechnology , measles , chemistry , genetics , in vitro , vaccination , organic chemistry
The expression of intercellular adhesion molecule 1 (ICAM‐1) by endothelial cells is important for the regulation of adhesion and transendothelial migration of a variety of leukocytes that express the integrins lymphocyte function‐associated antigen 1 (LFA‐1) and/or Mac‐1. Here, we demonstrate strain‐specific differences in the ability of measles virus (MV) to induce ICAM‐1 expression. The vaccine strain Moraten (Mor) rapidly induced high levels of ICAM‐1 mRNA and protein expression, whereas the vaccine strain CAM‐70 and the Edmonston wild type (Ed‐wt) strain were far less effective, even when they were used at very high multiplicities of infection (MOIs). Strain‐specific differences in the induction were not a consequence of differences in the ability to infect ECs. Furthermore, induction of ICAM‐1 by Mor was not dependent on de novo expression of MV or cellular proteins. Dual‐immunofluorescence analysis indicated that there was no association between the expression of either MV nucleocapsid or hemagglutinin protein and the induction of ICAM‐1 expression. Some human umbilical vein endothelial cells (HUVECs) that expressed high nucleocapsid protein in response to either Mor or CAM‐70 failed to express elevated ICAM‐1, whereas some HUVECs that were incubated with Mor expressed high ICAM‐1 prior to expression of MV nucleocapsid protein. Strain‐specific differences in the ability of Mor and CAM‐70 to induce ICAM‐1 correlated with their ability to activate the latent transcription factor NF‐κB. These studies suggest a preexisting component of MV particles that leads to strain‐specific differences in the activation of NF‐κB and the induction of ICAM‐1 gene expression. J. Med. Virol. 57:9–16, 1999. © 1999 Wiley‐Liss, Inc.